Once transcription is initiated, the DNA double helix unwinds and RNA polymerase reads the template strand, adding nucleotides to the 3′ end of the growing chain (Figure 2b). At a temperature of ...

DNA is essentially a storage molecule. It contains all of the instructions a cell needs to sustain itself. These instructions are found within genes, which are sections of DNA made up of specific ...

Here, we will focus on eukaryotic cells. Transcription is the synthesis of any type of complimentary RNA from a DNA template: note, several types of RNA can be encoded by a DNA strand. Here, we focus ...

Using the DNA strand as template, a long chain of nucleotides are formed. This is called transcription. Initiation of transcription begins with the binding of the enzyme to a promoter sequence in ...

As the DNA template strand moves through the RNA polymerase it builds an RNA strand that is complimentary to the transcribed DNA strand. 3. Termination is the final step of transcription. Once RNA ...

The team proposes that the rate-limiting step in transcription may be the positioning of the DNA template strand within the active site of the RNAP enzyme. This step involves overcoming ...

random primers and/or gene-specific priming to reverse transcribe RNA to cDNA. The kit contains all required components for first-strand cDNA synthesis except RNA template and gene-specific primers.