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The influence of blind spots on genomic recovery by the panel probes was examined in the genomic libraries. The team also evaluated the coverage of the probe with respect to reference sequences ...
San Diego-based Illumina and the San Diego Zoo Wildlife Alliance hope decoding individual DNA sequences will help minimize the risk facing koalas.
The data show fourplex sequencing of an enriched fraction of genomic DNA. Target capture was performed using a 1.2 Mb xGen Lockdown Probe panel and sequences were run on an Illumina MiSeq® platform.
One method that allows for targeted sequencing is called hybridization capture-based enrichment, which uses short pieces of nucleic acids called oligonucleotides as capture probes.
Further, they aligned primer and probe sequences and their reverse complements in the seven real-time PCR assays against 1,779 MPXV genomes and computed the percentage of genomes with a 100% match.
This molecular clamp consists of a synthetic, sequence-specific Xeno-nucleic acid (XNA) probe. It is called QClamp™.
After adding the probes to sample extracts, the target aDNA binds to the probes and is then "fished out" from the massive amount of environmental DNA. This technology is widely used in ancient ...
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